甘蔗花叶病研究进展

根据国内外的研究现状，综述了甘蔗花叶病的症状与危害、致病病毒、发病规律、病毒检测及防治措施等方面的研究进展．并提出了今后的研究方向和发展目标。     

网络环境下的编目工作创新研究

编目工作始终是图书馆业务工作中十分重要的基础性工作。网络环境下，编目对象的多元化、数字化，用户信息行为和方式的改变，书目中心的蓬勃发展使得编目工作面临被边缘化的危险。就此提出实现编目工作创新的几点建议，以使其能够更好地为广大用户服务。     

利用抑制消减杂交技术分离辣椒细胞质雄性不育育性恢复相关EST

 以辣椒(Capsicum annuum L. ) 细胞质雄性不育系23A、121A, 和其相应的近等基因恢复系23C、121C为试验材料, 利用抑制消减杂交( SSH) 技术成功构建了CMS恢复基因诱导表达的消减cDNA文库。结合高密度点阵膜杂交差异筛选, 获得了282个阳性克隆。通过测序, 除去重复序列共得到175个Unique ESTs。在GenBank上进行BLAST分析, 55个EST片段未找到对应的同源序列, 可能代表了新基因;120个EST片段找到了对应的同源序列, 包括103个已知功能基因和17个未知功能基因。按照MIPS功能分类法, 将其分为14个功能组, 涉及代谢、胁迫应答、蛋白活性、转录因子、信号转导等多方面的功能。     

Differentiation of human bone marrow-derived stem cells into insulin-producing cells in vitro and in vivo

AIM: To explore the possibility of differentiating functional insulin-producing cells from human BM-derived stem cells. METHODS: Mesenchymal stem cells were isolated from human bone marrow. Then these cells were induced with epidermal growth factor, β-mercaptoethanol and high concentration of glucose. The gene expression related to islet β cells was detected by RT-PCR. Insulin in the treated cells was examined by immunocytochemistry. In addition, the levels of insulin secretion and glucose-stimulated insulin release were examined by microparticle enzyme immunoassay. Finally, the induced cells were implanted into the right renal subcapsular space of diabetic mice. Blood glucose levels were monitored 16 d after implantation. The right kidneys of the treated mice were harvested for immunohistochemistry. RESULTS: The key genes related to pancreatic β cells had been confirmed to express by PCR and insulin was detected by immunocytochemistry in differentiated human BM-derived stem cells induced by high glucose, which responded to glucose challenge. Furthermore, implantation of the cells in renal subcapsular space was able to lower the glucose levels in hyperglycemic mice. After 16 days, the implanted cells were determined still to be insulin positive cells by immunohistochemistry. CONCLUSION: These results indicate human BM-derived stem cells are capable of differentiating into functional insulin-producing cells and may represent a pool of cells for the treatment of diabetes.     

砂土中粘土夹层对入渗过程影响的试验分析

对水在高渗透性砂内入渗过程中,其内部的低渗透性粘土夹层对入渗过程的影响进行了室内垂直一维入渗试验。通过分析定性得出粘土夹层对入渗过程的影响体现为减渗作用,表现为粘土夹层将均质粗砂中的非线性入渗过程变为夹层内的线性入渗过程,使夹层范围的入渗为一个具有较小入渗率和较小湿润锋推进速度的稳渗阶段。     

基于核K—均值聚类算法的植物叶部病害识

针对植物叶部病害图像的特点,首先对采集到的玉米病害彩色图像采用矢量中值滤波法去除噪声,然后提取玉米病叶彩色图像的纹理特征和颜色特征作为特征向量,利用Mercer核,把输入空间的样本映射到高维特征空间进行K-均值聚类以及植物病害识别.试验涉及的4种玉米病害识别正确率达82.5%,核K-均值聚类方法适合玉米叶部病害分类.     

莲草直胸跳甲成虫产卵选择性及叶位与幼虫发育适合度的关系

采用室内自由选择法和发育测定法研究莲草直胸跳甲(Agasicles hygrophila)成虫对喜旱莲子草(Alternanthera philoxeroides)叶位的产卵选择性及后代发育与不同叶位叶片的适合度.结果表明:(1)莲草直胸跳甲成虫嗜好在中部叶片的叶背产卵,以在第2对叶上的选择系数最高,选择率为35.18%,与第1对叶上的28.03%差异不显著,但显著高于对其他叶位的选择系数;(2)相对于在叶表进行取食,初孵幼虫显著趋向在叶背,且这样取食的1龄幼虫存活率高、发育历期显著缩短,这与雌虫选择在叶背产卵的习性相符,说明其选择有一定生物学意义,即有利于后代发育;(3)叶龄对莲草直胸跳甲各龄幼虫发育有显著影响,叶位越低,各龄幼虫的发育历期越长,但存活率上以取食第1叶位的为最高.     

24％溴虫腈·辛硫磷乳油对家蚕残毒试验

桑园治虫用药一直移用农田农药，安全性差，家蚕中毒事故频发；近年虽有一些桑园专用农药推出，但由于数量少，使用频率高，促成了害虫耐药性迅速提高，造成多种药物防效明显下降；开发桑园专用新农药，对桑园害虫控制和抗性治理都有积极意义。     

完善沈阳市"农业110"科技服务网络建设促进农民增收

沈阳市"农业110"科技服务网络的诞生是提高农业科技知识普及率的具体体现.7年来,在沈阳市委、市政府的正确领导下,沈阳市农业技术推广站通过利用"农业科普热线讲座"、"农业110"咨询服务电话、"农业科技服务队"三种服务形式,传授先进科学技术,解决农民的疑难问题,提高他们的技术水平,促进农业增效,农民增收,繁荣农村经济.